IDENTIFIKASI BAKTERI Escherichia coli, Enterobacter cloacae, Haemophillus influenza, Pseudomonas aeruginosa PADA SPUTUM PASIEN PENDERITA TUBERKULOSIS DENGAN METODE MULTIPLEX RT-PCR

Izzati Meiziza Juzda, Izzati (2025) IDENTIFIKASI BAKTERI Escherichia coli, Enterobacter cloacae, Haemophillus influenza, Pseudomonas aeruginosa PADA SPUTUM PASIEN PENDERITA TUBERKULOSIS DENGAN METODE MULTIPLEX RT-PCR. Skripsi thesis, Universitas Perintis Indonesia.

	Text ARTIKEL IZZATI MEIZIZA JUZDA PDF.pdf Restricted to Repository staff only Download (731kB)
	Text SKRIPSI BAB 1 & 5 IZZATI MEIZIZA JUZDA.pdf Download (846kB)
	Text SKRIPSI FULL IZZATI MEIZIZA JUZDA .pdf Restricted to Repository staff only Download (3MB)

Abstract

Tuberculosis (TB) remains a major public health concern in Indonesia. One of the frequent complications is secondary respiratory infection caused by other bacteria, such as Haemophilus influenzae, Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae. This study aimed to detect co-infecting pneumonia bacteria in TB patients using the Multiplex Real-Time PCR method with the Crown Lab® Pneumoplex 3.0 kit. Sputum samples were collected from clinically confirmed TB patients. DNA extraction was followed by amplification of target genes using a CFX96 Real-Time PCR instrument. The results showed simultaneous detection of multiple bacteria with varied Ct values. Haemophilus influenzae was the most frequently detected pathogen, followed by E. coli and P. aeruginosa. The Multiplex RT-PCR method proved to be sensitive and specific, enabling rapid and efficient detection of co-infection in TB patients. Therefore, this molecular approach supports more accurate diagnosis and targeted therapy. Keyword : Tuberkulosis, ko-infeksi, pneumonia, multiplex PCR, Crown Lab Pneumoplex 3.0.

Actions (login required)

View Item