DETEKSI GEN JAMUR Candida albicans DARI ISOLAT KEPUTIHAN MENGGUNAKAN METODE Polymerase Chain Reaction (PCR)

Sulestia Cahyani, Sulestia Cahyani (2025) DETEKSI GEN JAMUR Candida albicans DARI ISOLAT KEPUTIHAN MENGGUNAKAN METODE Polymerase Chain Reaction (PCR). Skripsi thesis, Universitas Perintis Indonesia.

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Abstract

Candida albicans is an opportunistic fungus that is often the main cause of genital tract infections, particularly vaginal discharge. Vaginal discharge occurs due to an imbalance of the normal vaginal flora, which triggers the overgrowth of Candida albicans and leads to symptoms such as greenish-white mucus, itching, and unpleasant odor. This study aimed to detect the presence of specific genes of Candida albicans from vaginal discharge isolates using the Polymerase Chain Reaction (PCR) method. The type of research used was descriptive with a cross sectional design. A total of 10 samples were collected from women experiencing vaginal discharge and isolated on Sabouraud Dextrose Agar (SDA) medium. Initial identification was carried out macroscopically and microscopically, followed by amplification of the ITS (Internal Transcribed Spacer) gene specific for Candida albicans using ITS1 and ITS4 primers. From the 10 isolates, 3 were identified as positive for Candida albicans. Further analysis using PCR showed that only 1 isolate produced a clear DNA band at 600 bp. The sensitivity value obtained was 33.3%, indicating that the PCR method was not fully optimal, possibly due to poor DNA quality. Keywords : Candida albicans, Vaginal discharge, Polymerase chain reaction (PCR)

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